BEAVER Biomedical Engineering Co., LTD.

BEAVER Biomedical Engineering Co., Ltd.

Manufacturer from China
Verified Supplier
4 Years
Home / Products / Magnetic Beads Protein Purification /

30 - 150 μm Magnetic Beads Protein Purification 10% Volume Ratio 100 mL

Contact Now
BEAVER Biomedical Engineering Co., LTD.
Visit Website
City:suzhou
Country/Region:china
Contact Person:MrsElaine
Contact Now

30 - 150 μm Magnetic Beads Protein Purification 10% Volume Ratio 100 mL

Ask Latest Price
Video Channel
Brand Name :BeaverBeads
Model Number :70804-100
Certification :COA
Place of Origin :China
MOQ :5 mL / bottle
Price :Negotiation
Payment Terms :L/C, T/T
Supply Ability :850 L/month
Delivery Time :5-8 days
Packaging Details :5mL/100mL/500mL
Product Name :Magrose Protein A Magbeads
Application :Protein Purification
Shelf life :2 years
Usage :Medical Research
Storage :2~8℃ sealed storage
Package :5mL/100mL/500mL
Concentration :10% (v/v)
Specification :100 mL, 10% (v/v), 30-150 μm
more
Contact Now

Add to Cart

Find Similar Videos
View Product Description

Magrose Protein A Magbeads for Protein Purification 30-150 μm, 10% (v/v)

1, Description:

BeaverBeads™ Protein A, Protein G are superparamagnetic beads covalently coated with recombinant Protein A or Protein G that contains Fc binding domains for the purification of monoclonal or polyclonal antibodies from cell lysate, blood plasma, ascites and tissue culture supernatant, and for antibody immobilization studies. This product is micron magnetic beads, skilled operation can complete the antibody adsorption process within 15 min, and complete the process of antibody purification within 30 min. It can be used for antibody fixation and other related studies. By referring the attached Table 1 users can select the categories of magnetic beads according to the source and subtype of the target antibody, and compare the affinity of Magrose Protein A and Magrose Protein G beads with different antibodies.

2, Product Information:

Product Name
Magrose Protein A
Bead Size
30~150 μm
Beads Concentration
10% (v/v)
Ligand
Protein A
Medium
Magrose
Antibody Bonding Quantity
25~30 mg Human IgG/mL Gel
Storage Temperature
2~8℃
Binding/Washing Buffer
PBST (pH 7.2~7.4): 137 mM NaCl,2.7 mM KCl, 10 mM
Na2HPO4, 2.0 mM KH2PO4, 0.1% Tween-20
Elution buffer
100 mM Gly, 0.1% Tween-20, pH 2.5
Neutrilization buffer
1.0 M Tris-HCl, pH 9.0
Storage buffer
PBST (includes 0.05% NaN3)
Shelf life
12 months

3, Feature:

1. Highly effective antibody binding capacity and ultra-low non-specific adsorption performance

2. Time saving, simple and mild

3. High product stability

4. Antibody elution system is closer to neutral

5. Very low protein A ligand off rate

6. Reusable

4, FAQ:

Q1: How to avoid possible aggregation of magnetic beads during storage or use?
A1: Beads should be stored at 2~8℃, and avoid for irreversible aggregation due to contamination or drying. Magnetic beads aggregation at low pH elution buffer is a normal phenomenon, and it does not affect the normal use of magnetic beads. In Binding/Washing buffer and Elution buffer adding with the final concentration of 0.1% (v/v) nonionic detergent (such as NP-40, Tween-20 or Triton X-100) can effectively prevent the beads
aggregation. The magnetic beads in low pH elution can be washed to neutral with Binding/Washing buffer and Elution buffer, and process with 2 min ultrasonic water bath. It can restore the magnetic beads to homogeneity, and the above treatments had no effect on the antibody binding efficiency with magnetic beads.
Q2: How to solve magnetic bead adhere to tube wall?
A2: It is recommended to use the consumables with low adsorption rate. In addition, adding 0.01%~0.1%(v/v) nonionic detergent (such as NP-40, Tween-20 or Triton X-100) to the buffer solution can effectively reduce the adhesion of beads on tube wall.
Inquiry Cart 0