BEAVER Biomedical Engineering Co., LTD.

BEAVER Biomedical Engineering Co., Ltd.

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1000 mL NHS Activated Magnetic Beads 30 - 150 μm, 20% Volume Ratio

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BEAVER Biomedical Engineering Co., LTD.
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Country/Region:china
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1000 mL NHS Activated Magnetic Beads 30 - 150 μm, 20% Volume Ratio

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Brand Name :BeaverBeads
Model Number :70702-1000
Certification :COA
Place of Origin :China
MOQ :1 mL / bottle
Price :Negotiation
Payment Terms :T/T, L/C
Supply Ability :850 L/month
Delivery Time :5-8 days
Packaging Details :1mL/5mL/50mL/1000mL
Product Name :Agarose NHS Magbeads Preactivated
Bead diameter :30-150 μm
Concentration :20% (v/v)
Magnetic core :Fe3O4
Package :1mL/5mL/50mL/1000mL
Specification :1000 mL, 20% (v/v), 30-150 μm
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Agarose NHS Magbeads Preactivated N - Hydroxy Succinimide Nanoparticle​ 30 - 150 μm, 20% (v/v)

1, Description:

The NHS magnetic beads are superparamagnetic beads with activated groups of N_Hydroxysuccinimide on the surface, which can react with primary amines on desired ligands and bind them covalently with amide linkage to the beads. The ligand can be various molecules including antigen, antibody and other proteins. Compared with the traditional carboxyl, amino magnetic beads, the NHS group of magnetic beads don’t need to activate EDC/NHS or Glutaraldehyde, simply dissolving the amino-containing bio-ligand in the coupling buffer, at room temperature, mixing with proteins and the NHS magnetic beads for only 1-2 hours, the bio-ligand can be coupled to the magnetic beads. The advantages include easy operation, mild coupling conditions and coupling fast and efficient. The magnetic bead coupling process must be carried out in a buffer solvent free of any amino. This method can be operated manually or automatically.

2, Introduction:

Product Name Agarose NHS Magbeads Preactivated
Mean particle size 30-150 μm
Magnetic core Fe3O4
Shell Agarose
Magnetic superparamagnetism
Ligand content ~50 μM/mL gel
Binding ability 20~30 mg rabbit IgG/mL beads
Preservation fluid 20% (v/v) ethanol solution
Operation Automate/manual
* water average particle size, Malvern Nano determination

3, FEATURES:

  • 1. Simple, without activation, can be directly covalently coupled with the biological ligand;

  • 2. Efficient, bio-ligand coupling efficiency of up to 90% or more, much higher than the carboxyl beads, while the ligand with a higher binding capacity;

  • 3. Fast, 1 ~ 2h to complete the bio-ligand coupling;

  • 4. Mild, room temperature or 4 ℃ coupling, coupling system pH 5 ~ 9;

  • 5. Stable, the formation of a stable amide bond, to prevent the ligand off.

  • 6. Good biocompatibility, reduced nonspecific adsorption.

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